Gene expression profiling of inflammatory cytokines in esophageal biopsies of different phenotypes of gastroesophageal reflux disease: a cross-sectional study

Abstract Background The clinical endoscopic phenotypes of gastroesophageal reflux disease (GERD) are classified as Barrett's esophagus (BE), erosive esophagitis (EE) and non-erosive (NERD). NERD is subclassified abnormal acid exposure (AAE) normal (NAE) based on pH monitoring study results. aim this was to characterize genes involved in the pathophysiology immune response GERD. Methods This an observational cross-sectional study. All patients with BE, EE, AAE, NAE a control group were subjected superior endoscopy (with biopsies esophageal mucosa). Relative mRNA quantification cytokine target conducted by quantitative Polymerase Chain Reaction (RT-qPCR). Changes expression associated inflammation assessed for each phenotype. Statistical analysis differential gene performed using Mann–Whitney U non-parametric test. A p value < 0.05 considered significant. Results total 82 included divided into following groups: Group 16 (19.51%); 23 (28.04%); 13 (15.86%); Control Group, 17 (20.73%). Compared group, BE exhibited increased IL-8 ( 0.05) levels IL-10, MMP-3, MMP-9. Patients EE IL-1B, IL-6 IL-10 0.05), AAE IL-6, IFN-? TNF-? 0.05). IL-1B compared Conclusion demonstrates mediators mucosa different GERD phenotypes. could be useful differentially diagnose phenotype biopsies.